Silver nanoparticles induce oocyte maturation in zebrafish danio. Interestingly, preliminary studies with a zebrafish ortholog of the atonal1 gene unpublished, which in mammals directs differentiation of goblet, enteroendocrine and paneth cell intestinal lineages from a multipotent progenitor yang et al. In early zebrafish development between 16 and 24 hpf, mir145 is expressed ubiquitously at very low levels fig. Proceedings of the national academy of sciences of the united. Results loss of mir145 leads to defects in heart and gut development.
Microcystinlr promotes zebrafish danio rerio oocyte in vivo. Lh signaling is required for oocyte maturation in fish and other. Here we developed a new experimental approach for direct analysis. The zebrafish as a model to study intestinal inflammation. Given that several gut functions and immune genes are conserved between zebrafish and mammals, the zebrafish is an interesting model organism to investigate fundamental processes underlying intestinal inflammation and injury cheesman and guillemin, 2007, yang et al. The zebrafish intestine is a simple tapered tube that is folded into three. Loss of mir145 leads to defects in heart and gut development. The role of micrornas in modulating gut epithelial or smooth muscle differentiation is currently not known. For gene knockout studies, ab strain zebrafish danio rerio were. The rapid specification and differentiation of the embryonic zebrafish gut is essential to provide contractility for the digestion of food. S1a with increasingly specific expression in the heart, ear, and pharyngeal arches between 24 and 72 hpf. Igfs mediate the action of lh on oocyte maturation in zebrafish. In the present study, we used the zebrafish ovarian follicle as a model to. Here we show that the microrna mir145 is strongly expressed in zebrafish gut smooth muscle and regulates its development.
Pdf mir145 directs intestinal maturation in zebrafish. Although we cant provide direct evidence to approve the quality of. In early zebrafish development between 16 and 24 hpf, mir145 is. We show that alkaline phosphatase activity is completely absent in mir145 morphants at 96 hpf, also indicating a lack of functional maturation fig. Intestinal growth and differentiation in zebrafish. The evolution of the adaptive immune system coincided with the emergence of jawed vertebrates, far in advance of the divergence of fishes from other vertebrates. Morphological and molecular evidence for functional organization. Similarly, alkaline phosphatase activity is an indicator of gut epithelial maturation and normally appears in the zebrafish intestinal lumen by 72 hpf. Adaptive immune maturation in zebrafish is an active research topic within the. Microcystinlr promotes zebrafish danio rerio oocyte in vivo maturation by activating erk12mpf signaling pathways, and camp is. Here we developed a protocol to raise microbefree zebrafish larvae fed on microbefree live food. Thus, the adaptive and innate branches of the immune system are remarkably conserved across jawed fishes teleosts and. This expression pattern was also confirmed by direct detection of. One of the early choices in the differentiation of epithelial cells is the distinction of whether a cell will become an enterocyte or a secretory cell fig.
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